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KMID : 0380620120440030317
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Korean Journal of Food Science and Technology
2012 Volume.44 No. 3 p.317 ~ p.323
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Cultivated Orostachys japonicus Induces Apoptosis in Human Colon Cancer Cells
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Kim Jae-Yong
Jung Eun-Jung
Won Yeong-Seon
Lee Ju-Hye
Seo Kwon-Il
Shin Dong-Young
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Abstract
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This study was performed to elucidate the anticancer activities and the mechanism of chloroform fractions from cultivated Orostachys japonicus (CFCOJ) in human colon cancer cells. CFCOJ markedly decreased viable cell numbers in both a dose-dependent and time-dependent manner within SW480 cells. Cell death induced by CFCOJ increased cell populations in the sub-G1 phase, as well as the formation of apoptotic bodies, nuclear condensation, and induced DNA fragmentation. CFCOJ-induced apoptosis was associated with the activation of initiator caspase-8 and -9, as well as the effector caspase-3. CFCOJ also stimulated Bid cleavage, indicating that the apoptotic action of caspase-8-mediated Bid cleavage leads to the activation of caspase-9. CFCOJ increased the expression of the proapoptotic protein, Bax, and decreased the expression of the antiapoptotic protein, Bcl-2. These results indicate that CFCOJ exert anticancer effects on human colon cancer SW480 cells through a caspase-dependent apoptotic pathway.
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KEYWORD
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Orostachys japonicus, human colon cancer cell, anticancer activity, apoptosis
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